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CELSR2 Polyclonal antibody

CELSR2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19940-1-AP

Synonyms

EGF-like protein 2, EGFL2, EGF like protein 2, CDHF10, Cadherin family member 10

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using CELSR2 Polyclonal antibody (19940-1-AP)

    IHC staining of mouse brain using 19940-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19940-1-AP (CELSR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CELSR2 Polyclonal antibody (19940-1-AP)

    IF Staining of SH-SY5Y using 19940-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CELSR2 antibody (19940-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19940-1-AP targets CELSR2 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila)
Calculated Molecular Weight 318 kDa
GenBank Accession NumberNM_001408
Gene Symbol CELSR2
Gene ID (NCBI) 1952
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CELSR2, also named CDHF10, EGFL2, KIAA0279, and MEGF3, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. It is a receptor that may have an important role in cell/cell signaling during nervous system formation.

Protocols

Product Specific Protocols
IHC protocol for CELSR2 antibody 19940-1-APDownload protocol
IF protocol for CELSR2 antibody 19940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols