Desmocollin 2 Polyclonal antibody

Desmocollin 2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 13876-1-AP

Synonyms

DSC2, Desmocollin-3, Desmocollin-2, Desmocollin 3, CDHF2

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Validation Data Gallery

• 

  WB analysis using 13876-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus using 13876-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human oesophagus using 13876-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of mouse heart using 13876-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13876-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 13876-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 13876-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 13876-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 13876-1-AP (Desmocollin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 13876-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Desmocollin 2 antibody (13876-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 13876-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Desmocollin 2 antibody (13876-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human oesophagus tissue, human skin cancer tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

13876-1-AP targets Desmocollin 2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Desmocollin 2 fusion protein Ag4956
• Full Name: desmocollin 2
• Calculated Molecular Weight: 100 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC063291
• Gene Symbol: Desmocollin 2
• Gene ID (NCBI): 1824
• RRID: AB_2877983
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Desmocollin 2 (DSC2) is a member of the desmocollin subfamily of the cadherin superfamily. The desmocollins (DSC1-3), along with the desmogleins (DSG1-4), are found primarily in epithelia and cardiac muscle where they constitute the adhesive proteins of the intercellular desmosome junctions and are required for cell adhesion and desmosome formation. Mutations in DSC2 gene are associated with arrhythmogenic right ventricular dysplasia-11.

Publications

Species	Application	Title
human	IF	Dev Cell; Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model.; Authors - Sally Dabelsteen
mouse	WB	Sci Total Environ; Environmental cadmium impairs blood-testis barrier via activating HRI-responsive mitochondrial stress in mice.; Authors - Guo-Xiang Zhou
human	IF	Br J Cancer; Mapping of truncated O-glycans in cancers of epithelial and non-epithelial origin.; Authors - Troels Boldt Rømer
human	WB	Bioconjug Chem; Transforms of Cell Surface Glycoproteins Charge Influences Tumor Cell Metastasis via Atypically Inhibiting Epithelial-Mesenchymal Transition Including Matrix Metalloproteinases and Cell Junctions; Authors - Mingzhe Wang
human	IHC	Mol Ther Oncolytics; Exosomal miR-205-5p enhances angiogenesis and nasopharyngeal carcinoma metastasis by targeting desmocollin-2.; Authors - Wenjuan Yang
human	WB,IHC,IF	BMC Cancer; Desmoglein 2 and desmocollin 2 depletions promote malignancy through distinct mechanisms in triple-negative and luminal breast cancer; Authors - Ji-Yuan Han ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jane (Verified Customer) (02-03-2022)

One specific band was found around 120kDa, however, the signal is weak so need to use lower dilution

• Applications: Western Blot
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Heart tissue

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