CoraLite® Plus 488-conjugated CEBPB Recombinant antibody

CEBPB Uni-rAb^TM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240836G6

Cat no : CL488-83791-6

Synonyms

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-83791-6

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 CEBPB antibody (CL488-83791-6, Clone: 240836G6 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83791-6 is CEBPB Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CEBPB fusion protein Ag20073
• Full Name: CCAAT/enhancer binding protein (C/EBP), beta
• Calculated Molecular Weight: 345 aa, 36 kDa
• Observed Molecular Weight: 42 kDa, 46 kDa
• GenBank Accession Number: BC007538
• Gene Symbol: CEBPB
• Gene ID (NCBI): 1051
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CCAAT/enhancer-binding protein beta (CEBPB), also known as LAP, is a important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. It specifically binds to an IL-1 response element in the IL-6 gene. CEBPb mRNAs possess alternative translation-initiation codons, which result in the formation of truncated forms of the protein.Three variants of CEBPBs have been detected: a 46 kDa full-length liver-enriched transcription-activating protein (LAP1), a 42 kDa LAP2 and a 20 kDa liver-enriched transcription-inhibitory protein (LIP). (PMID:18820298).