CEBP Alpha/CEBPA Recombinant antibody, PBS Only (Detector)

CEBP Alpha/CEBPA Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242101C10

Cat no : 84668-2-PBS

Synonyms

CEBP alpha, CEBPA, CEBP, CCAAT/enhancer-binding protein alpha, C/EBPα

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Validation Data Gallery

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  WB analysis using 84668-2-RR (same clone as 84668-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84668-2-RR (CEBP Alpha/CEBPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84668-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 84668-2-RR (same clone as 84668-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CEBP Alpha/CEBPA Recombinant antibody (84668-2-RR, Clone:242101C10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84668-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 84668-2-RR (same clone as 84668-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CEBP Alpha/CEBPA Recombinant antibody (84668-2-RR, Clone:242101C10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84668-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01479-1

  Cytometric bead array standard curve of MP01479-1, CEBP Alpha/CEBPA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84668-1-PBS. Detection antibody: 84668-2-PBS. Standard: Ag29947. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84668-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84668-2-RR against Human CEBP Alpha/CEBPA were performed. The affinity constant is 1.15 nM.

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Product Information

84668-2-PBS targets CEBP Alpha/CEBPA as part of a matched antibody pair:

MP01479-1: 84668-1-PBS capture and 84668-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CEBP Alpha/CEBPA fusion protein Ag29947
• Full Name: CCAAT/enhancer binding protein (C/EBP), alpha
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: NM_004364
• Gene Symbol: CEBPA
• Gene ID (NCBI): 1050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CEBPA and its isoforms play important roles in lineage determination and gene activation in a variety of cell types by activating transcription from lineage-specific promoters. CEBPA is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. In hematopoiesis, C/EBPa is a key factor in driving the development of myeloid cells interacting with a variety of factors, including c-Myc, PU.1, and microRNAs. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin which plays an important role in body weight homeostasis. CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Several pathways have been implicated as the means by which CEBPA mediates cell cycle arrest and proliferation, including p21, cyclin-dependent kinases and the E2F complex via c-Myc. The calcualted molecular weight of CEBPA is 38 kDa, but modified CEBPA is about 42 kDa (PMID: 19623175).