FcZero-rAb™ APC Anti-Human CEACAM5/8 (CD66b/e) Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocyte using APC-FcA98012

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul APC Anti-Human CEACAM5/8 (CD66b/e) Rabbit RecAb (APC-FcA98012, Clone: 230353B7), or 5 ul APC Rabbit IgG Isotype Control RecAb (APC-FcA98136, Clone: 240953C9). Cells were not fixed.

CEACAM5/8 (CD66b/e) FcZero-rAb^TM Recombinant Antibody for FC

Cat No. APC-FcA98012

Clone No.230353B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD66e, CEACAM5, CD66b, CEACAM8, 230353B7

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Tested Applications

• Positive FC detected in: human peripheral blood leukocyte

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-FcA98012 targets CEACAM5/8 (CD66b/e) in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CEACAM5/8 (CD66b/e) fusion protein Eg32133
• Full Name: carcinoembryonic antigen-related cell adhesion molecule 8
• Calculated Molecular Weight: 38 kDa
• GenBank Accession Number: BC026263
• Gene Symbol: CEACAM8
• Gene ID (NCBI): 1088
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) belong to the immunoglobulin superfamily of cell adhesion molecules. CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in controlling cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e mainly serves as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEACAM8, also known as CD66b, CGM6 or NCA-95, is a glycosylphosphatidylinositol (GPI)-linked glycoprotein expressed on granulocytes and is involved in regulating adhesion and activation of eosinophils (PMID: 8104998; 18056392; 18056392). This antibody is raised against 35-319 aa of human CEACAM8/CD66b and can cross-react with CEACAM5/CD66e.