CDK9 Polyclonal antibody

CDK9 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11705-1-AP

Synonyms

C 2k, CDC2L4, CDK9, Cell division protein kinase 9, CTK1, cyclin dependent kinase 9, PITALRE, TAK



Tested Applications

Positive WB detected inHEK-293 cells, human placenta tissue, Jurkat cells, NIH/3T3 cells
Positive IHC detected inhuman gliomas tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11705-1-AP targets CDK9 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK9 fusion protein Ag2318
Full Name cyclin-dependent kinase 9
Calculated Molecular Weight 372 aa, 43 kDa
Observed Molecular Weight 38-42 kDa, 55 kDa
GenBank Accession NumberBC001968
Gene Symbol CDK9
Gene ID (NCBI) 1025
RRIDAB_2077302
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243). CDK9 has two isoforms with the molecular mass of 42 kDa and 55 kDa, and the relative abundance of Cdk9(42kDa) and Cdk9(55kDa) changes in different cell types (PMID: 12706900, 15780980).

Protocols

Product Specific Protocols
WB protocol for CDK9 antibody 11705-1-APDownload protocol
IHC protocol for CDK9 antibody 11705-1-APDownload protocol
IF protocol for CDK9 antibody 11705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IP

Nat Commun

The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression.

Authors - Xinzhi Li
mouseWB

Nat Commun

Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH)

Authors - Xinzhi Li
humanWB

Nat Commun

Menin enhances c-Myc-mediated transcription to promote cancer progression.

Authors - Gongwei Wu
humanWB

Proteomics

A Simplified Thermal Proteome Profiling Approach to Screen Protein Targets of a Ligand.

Authors - Xiaolei Zhang
mouseIHC,WB

Clin Exp Pharmacol Physiol

Sevoflurane-induced P300 promotes neuron apoptosis via Sp1/SDK9 pathway

Authors - Xiaoqin Zhou

Chem Biol Drug Des

Synthesis, Structure-Activity Relationship Studies and Biological Evaluation of Novel 2,5-Disubstituted Indole Derivatives as Anticancer Agents.

Authors - Hongyu Hu