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CoraLite®555-conjugated CDK1 Monoclonal antibody

CDK1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2A11E4

Cat no : CL555-67575

Synonyms

CDC2, CDC28A, CDK1, Cell division protein kinase 1, Cyclin dependent kinase 1, DKFZp686L20222, p34 protein kinase

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated CDK1 Monoclonal antibody (CL555-67575)

    FC experiment of HeLa using CL555-67575

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human CDK1 (CL555-67575, Clone:2A11E4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-67575 targets CDK1 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CDK1 fusion protein Ag29018
Full Name cell division cycle 2, G1 to S and G2 to M
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC014563
Gene Symbol CDK1
Gene ID (NCBI) 983
RRIDAB_2919704
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.

Protocols

Product Specific Protocols
FC protocol for CL555 CDK1 antibody CL555-67575Download protocol
Standard Protocols
Click here to view our Standard Protocols