CDCA7L Recombinant antibody

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  WB analysis using 83682-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83682-4-RR (CDCA7L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of A431 using 83682-4-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug CDCA7L Recombinant antibody (83682-4-RR, Clone:240639A3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83682-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83682-4-RR against Human CDCA7L were performed. The affinity constant is 7.75 nM.

CDCA7L Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83682-4-RR

Clone No.240639A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

R1, Protein JPO2, JPO2, HR1, Cell division cycle-associated 7-like protein

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293T cells, HeLa cells, K-562 cells, MCF-7 cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83682-4-RR targets CDCA7L in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CDCA7L fusion protein Ag10944
• Full Name: cell division cycle associated 7-like
• Calculated Molecular Weight: 454 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC009352
• Gene Symbol: CDCA7L
• Gene ID (NCBI): 55536
• RRID: AB_3671283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96GN5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cell division cycle-associated 7-like protein (CDCA7L) is also known as HR1 and JPO2 and belongs to the JPO protein family. CDCA7L is identified as a target gene of c-Myc and is frequently dysregulated in multiple cancers. CDCA7L can complement the c-Myc transformation-defective mutant W135E and potentiate Myc-mediated transformation (PMID: 24141559, PMID: 37140023). CDCA7L translocates from cytoplasm to nucleus under dexamethasone induction. CDCA7L is widely expressed. CDCA7L has 4 isoforms produced by alternative splicing (refer to UniProt).