CDC6 Monoclonal antibody, PBS Only

CDC6 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6F12D2

Cat no : 66021-1-PBS

Synonyms

Cdc18 related protein, CDC18L, CDC6, CDC6 related protein, HsCDC18, HsCDC6, p62(cdc6)

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Validation Data Gallery

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  WB analysis using 66021-1-Ig (same clone as 66021-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66021-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 66021-1-Ig (same clone as 66021-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66021-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66021-1-Ig (same clone as 66021-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66021-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66021-1-Ig (same clone as 66021-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66021-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66021-1-Ig (same clone as 66021-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66021-1-Ig (CDC6 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66021-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66021-1-PBS targets CDC6 in WB, IF, Indirect ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CDC6 fusion protein Ag17958
• Full Name: cell division cycle 6 homolog (S. cerevisiae)
• Calculated Molecular Weight: 560 aa, 63 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC025232
• Gene Symbol: CDC6
• Gene ID (NCBI): 990
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CDC6, also named as CDC18L and HsCDC18, belongs to the CDC6/cdc18 family. It is involved in the initiation of DNA replication and functions as a regulator at the early steps of DNA replication. It also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5). MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human CDC6.