CDC6 Monoclonal antibody

CDC6 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB, ELISA, IF

Conjugate

Unconjugated

Publications

1

CloneNo.

6F12D2

Cat no : 66021-1-Ig

Synonyms

Cdc18 related protein, CDC18L, CDC6, CDC6 related protein, HsCDC18, HsCDC6, p62(cdc6)

---

Validation Data Gallery

• 

  WB analysis using 66021-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66021-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66021-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66021-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66021-1-Ig (CDC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 66021-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66021-1-Ig (CDC6 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

---

Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, ROS1728 cells, HEK-293T cells, Jurkat cell, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

66021-1-Ig targets CDC6 in WB, ELISA, IF applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CDC6 fusion protein Ag17958
• Full Name: cell division cycle 6 homolog (S. cerevisiae)
• Calculated Molecular Weight: 560 aa, 63 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC025232
• Gene Symbol: CDC6
• Gene ID (NCBI): 990
• RRID: AB_2881493
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC6, also named as CDC18L and HsCDC18, belongs to the CDC6/cdc18 family. It is involved in the initiation of DNA replication and functions as a regulator at the early steps of DNA replication. It also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5). MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human CDC6.

Publications

Species	Application	Title
human,mouse	IF	Mol Cancer; Deubiquitination of CDC6 by OTUD6A promotes tumour progression and chemoresistance; Authors - Jianfeng Cui