CDC40 Recombinant antibody

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  WB analysis using 84679-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84679-4-RR (CDC40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84679-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84679-4-RR against Human CDC40 were performed. The affinity constant is below 1 pM.

CDC40 Recombinant Antibody for WB, ELISA

Cat No. 84679-4-RR

Clone No.242034D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Pre mRNA processing factor 17, hPRP17, EHB3, EH binding protein 3, Cell division cycle 40 homolog

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Tested Applications

• Positive WB detected in: HEK-293T cells, HUVEC cells, HeLa cells, Jurkat cells, THP-1 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84679-4-RR targets CDC40 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CDC40 fusion protein Ag36151
• Full Name: cell division cycle 40 homolog (S. cerevisiae)
• Observed Molecular Weight: 65-67 kDa
• GenBank Accession Number: BC126114
• Gene Symbol: CDC40
• Gene ID (NCBI): 51362
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O60508
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC40 (cell division cycle 40), also known as EHB3. It is expected to be located in the nucleus, which is ubiquitinated in bone marrow and lymph node. The protein is required for pre-mRNA splicing as component of the activated spliceosome, and it plays an important role in embryonic brain development; this function does not require proline isomerization (PMID: 33220177; 33220177). The molecular weight of CDC40 is 65 kDa.