CDC27; APC3 Monoclonal antibody

CDC27; APC3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2F10G2

Cat no : 67239-1-Ig

Synonyms

CDC27, CDC27 homolog, APC3, APC 3, Anaphase-promoting complex subunit 3

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Validation Data Gallery

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  WB analysis of Jurkat using 67239-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67239-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 67239-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67239-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 67239-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67239-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 67239-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human lung cancer using 67239-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HEK-293 using 67239-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of K-562 using 67239-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CDC27; APC3 (67239-1-Ig, Clone:2F10G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, HSC-T6 cells, 4T1 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human lung cancer tissue
• Positive IF/ICC detected in: HEK-293 cells
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67239-1-Ig targets CDC27; APC3 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CDC27; APC3 fusion protein Ag25588
• Full Name: cell division cycle 27 homolog (S. cerevisiae)
• Calculated Molecular Weight: 92 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC011656
• Gene Symbol: CDC27
• Gene ID (NCBI): 996
• RRID: AB_2882518
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.