CD99 Recombinant antibody
CD99 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
241737H1
Cat no : 84580-4-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | THP-1 cells, Jurkat cells, HUVEC cells, U-937 cells, U-251 cells, MOLT-4 cells, human testis tissue |
Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
84580-4-RR targets CD99 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Recombinant Protein |
Full Name | CD99 molecule |
Calculated Molecular Weight | 19 kDa, 16 kDa, 17 kDa |
Observed Molecular Weight | 28 kDa, 32 kDa |
GenBank Accession Number | BC021620 |
Gene Symbol | CD99 |
Gene ID (NCBI) | 4267 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917).
Protocols
Product Specific Protocols | |
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WB protocol for CD99 antibody 84580-4-RR | Download protocol |
IF protocol for CD99 antibody 84580-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |