CD99 Recombinant antibody, PBS Only

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  WB analysis using 84580-4-RR (same clone as 84580-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84580-4-RR (CD99 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84580-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84580-4-RR (same clone as 84580-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84580-4-RR (CD99 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84580-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84580-4-RR (same clone as 84580-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84580-4-RR (CD99 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84580-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84580-4-RR (same clone as 84580-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84580-4-RR (CD99 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84580-4-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 84580-4-RR (same clone as 84580-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD99 antibody (84580-4-RR, Clone: 241737H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84580-4-PBS in a different storage buffer formulation.

CD99 {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84580-4-PBS

Clone No.241737H1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

MIC2, E2 antigen, CD99 molecule, CD99 antigen, 241737H1

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Product Information

84580-4-PBS targets CD99 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: CD99 molecule
• Calculated Molecular Weight: 19 kDa, 16 kDa, 17 kDa
• Observed Molecular Weight: 28 kDa, 32 kDa
• GenBank Accession Number: BC021620
• Gene Symbol: CD99
• Gene ID (NCBI): 4267
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P14209
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917).