Anti-Human CD98/SLC3A2 Rabbit Recombinant Antibody, PBS Only

• 

  FC experiment of human PBMCs using 98190-1-RR (same clone as 98190-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD98 Rabbit Recombinant Antibody (98190-1-RR, Clone: 241755D6) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98190-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98190-1-RR (same clone as 98190-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD98 Rabbit Recombinant Antibody (98190-1-RR, Clone: 241755D6) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98190-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98190-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98190-1-RR against Human CD98/SLC3A2 were performed. The affinity constant is 5.97 nM.

CD98/SLC3A2 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98190-1-PBS

Clone No.241755D6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD98, SLC3A2, MDU1, Amino acid transporter heavy chain SLC3A2, 4F2 cell-surface antigen heavy chain

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Product Information

98190-1-PBS targets CD98/SLC3A2 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
• Calculated Molecular Weight: 68 kDa
• GenBank Accession Number: NM_002394.5
• Gene Symbol: CD98
• Gene ID (NCBI): 6520
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P08195-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD98 is a 125-kDa type II membrane glycoprotein heterodimer composed of a 40-kDa non-glycosylated light chain (amino-acid transporter, encoded by the SLC7A5) and an 85-kDa glycosylated heavy chain (CD98hc, encoded by the SLC3A2) (PMID: 11507094). CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). Molecular orientation studies have demonstrated that the C-terminal tail of human CD98 (hCD98), which contains a PDZ-binding domain, is extracellular (PMID: 18625289).