Filter: AllWBIHCIF-P

WB analysis using 66766-1-Ig (same clone as 66766-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
WB analysis of pig colon using 66766-1-Ig (same clone as 66766-1-PBS)
pig colon tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
WB analysis of rabbit brain using 66766-1-Ig (same clone as 66766-1-PBS)
rabbit brain tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:9800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
WB analysis of U2OS using 66766-1-Ig (same clone as 66766-1-PBS)
U2OS cells were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
WB analysis of fetal human brain using 66766-1-Ig (same clone as 66766-1-PBS)
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
WB analysis of human kidney using 66766-1-Ig (same clone as 66766-1-PBS)
human kidney tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
WB analysis of pig brain using 66766-1-Ig (same clone as 66766-1-PBS)
pig brain tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
WB analysis of pig spleen using 66766-1-Ig (same clone as 66766-1-PBS)
pig spleen tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66766-1-Ig (same clone as 66766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66766-1-Ig (same clone as 66766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66766-1-Ig (same clone as 66766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66766-1-Ig (same clone as 66766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 66766-1-Ig (same clone as 66766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 66766-1-Ig (same clone as 66766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 66766-1-Ig (same clone as 66766-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 66766-1-Ig (same clone as 66766-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 66766-1-Ig (same clone as 66766-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66766-1-Ig (CD90 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 66766-1-Ig (same clone as 66766-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66766-1-Ig (CD90 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

CD90 Monoclonal antibody, PBS Only

CD90 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 66766-1-PBS

Clone No.2D7D11

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

THY1, CD90 / Thy1, 2D7D11, Thy 1 membrane glycoprotein, Thy 1 cell surface antigen

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66766-1-PBS targets CD90 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity	human, mouse, rat, pig, rabbit
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CD90 fusion protein Ag25603 Predict reactive species
Full Name	Thy-1 cell surface antigen
Calculated Molecular Weight	161 aa, 18 kDa
Observed Molecular Weight	28 kDa
GenBank Accession Number	BC065559
Gene Symbol	CD90/Thy1
Gene ID (NCBI)	7070
RRID	AB_2882112
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P04216
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.