CD90 Monoclonal antibody, PBS Only

CD90 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig, rat, rabbit, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D7D11

Cat no : 66766-1-PBS

Synonyms

CD90, CDw90, FLJ33325, Thy 1 antigen, Thy 1 cell surface antigen, Thy 1 membrane glycoprotein, THY1

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Validation Data Gallery

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  WB analysis using 66766-1-Ig (same clone as 66766-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig colon using 66766-1-Ig (same clone as 66766-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit brain using 66766-1-Ig (same clone as 66766-1-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:9800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66766-1-Ig (same clone as 66766-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig spleen using 66766-1-Ig (same clone as 66766-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 66766-1-Ig (same clone as 66766-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66766-1-Ig (same clone as 66766-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 66766-1-Ig (same clone as 66766-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66766-1-Ig (CD90 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66766-1-Ig (CD90 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66766-1-PBS targets CD90 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, pig, rat, rabbit, mouse samples.

• Tested Reactivity: Human, pig, rat, rabbit, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD90 fusion protein Ag25603
• Full Name: Thy-1 cell surface antigen
• Calculated Molecular Weight: 161 aa, 18 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC065559
• Gene Symbol: THY1
• Gene ID (NCBI): 7070
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.