CoraLite® Plus 488-conjugated CD9 Monoclonal antibody

• Featured Product
• KD/KO Validated

CD9 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

CloneNo.

4H7B9

Cat no : CL488-60232

Synonyms

5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, Cell and organelle markers, DRAP 27, Exosome marker, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29

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Validation Data Gallery

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  IF Staining of human lung cancer using CL488-60232

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 CD9 antibody (CL488-60232, Clone: 4H7B9 ) at dilution of 1:200.

• 

  IF Staining of human lung cancer using CL488-60232

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 CD9 antibody (CL488-60232, Clone: 4H7B9 ) at dilution of 1:200.

• 

  IF Staining of human breast cancer using CL488-60232

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488-conjugated CD9 antibody (CL488-60232, Clone: 4H7B9 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human lung cancer tissue, human breast cancer tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL488-60232 targets CD9 in IF-P applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD9 fusion protein Ag14529
• Full Name: CD9 molecule
• Calculated Molecular Weight: 228 aa, 25 kDa
• Observed Molecular Weight: 23-27 kDa
• GenBank Accession Number: BC011988
• Gene Symbol: CD9
• Gene ID (NCBI): 928
• RRID: AB_2919239
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.

Publications

Species	Application	Title
human	IF	Front Microbiol; Enterovirus 71 non-structural protein 3A hijacks vacuolar protein sorting 25 to boost exosome biogenesis to facilitate viral replication; Authors - Zhihui Ruan