PE Anti-Human CD8b Rabbit Recombinant Antibody

CD8b Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

241326A7

Cat no : PE-98103

Synonyms

CD8, 241326A7, Ly3, Leu2, CD8B1

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Validation Data Gallery

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  FC experiment of human PBMCs using PE-98103

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD3 and 5 ul PE Anti-Human CD8b Rabbit Recombinant Antibody (PE-98103, Clone: 241326A7) or Isotype Control. Cells were not fixed.

• 

  FC experiment of human PBMCs using PE-98103

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD3 and 5 ul PE Anti-Human CD8b Rabbit Recombinant Antibody (PE-98103, Clone: 241326A7) (red) or Isotype Control (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-98103 targets CD8b in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: CD8b molecule
• Calculated Molecular Weight: 243 aa, 27 kDa
• GenBank Accession Number: BC100913
• Gene Symbol: CD8B
• Gene ID (NCBI): 926
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD8b (T-cell surface glycoprotein CD8 beta chain) is an integral membrane glycoprotein that forms disulfide-linked heterodimers with CD8a (CD8 alpha chain). The CD8 alpha/beta heterodimer is the predominant CD8 complex expressed on the cell surface (PMID: 1534146; 2111591). CD8 is a transmembrane glycoprotein predominantly expressed on cytotoxic T cells and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells.