FcZero-rAb™ Biotin Anti-Human CD8a (OKT8) Rabbit IgG Recombinant Antibody

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  FC experiment of human PBMCs using Biotin-FcA65506

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD8a (OKT8) Rabbit IgG RecAb (Biotin-FcA65506, Clone: OKT8) or Biotin Rabbit IgG Isotype Control RecAb (Biotin-FcA98136, Clone: 240953C9), and PE-conjugated streptavidin. Cells were then stained with FcZero-rAb™ APC Anti-Human CD4 Rabbit Recombinant Antibody (APC-FcA98042, Clone: 240427E12). Cells were not fixed.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD8a FcZero-rAb^TM Recombinant Antibody for FC

Cat No. Biotin-FcA65506

Clone No.OKT8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD8, CD8a molecule, Leu2, MAL, OKT8

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA65506 targets CD8a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Human thymocytes
• Full Name: CD8a molecule
• Calculated Molecular Weight: 235 aa, 26 kDa
• GenBank Accession Number: BC025715
• Gene Symbol: CD8A
• Gene ID (NCBI): 925
• ENSEMBL Gene ID: ENSG00000153563
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T-cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).