APC-Cyanine7 Anti-Human CD8a (Hit8a)

CD8a Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

APC-Cyanine7 Fluorescent Dye

CloneNo.

Hit8a

Cat no : AY7-65113

Synonyms

CD8, p32, MAL, Leu2, Hit8a

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Validation Data Gallery

• 

  FC experiment of human PBMCs using AY7-65113

  1x10^6 human PBMCs were surface stained with 5 ul APC-Cyanine7 Anti-Human CD8a (Hit8a) (AY7-65113, Clone: Hit8a) or unstained. Cells were co-stained with CoraLite® Plus 488 Anti-Human CD3 (UCHT1) (CL488-65151, Clone: UCHT1). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

AY7-65113 targets CD8a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD8a molecule
• Calculated Molecular Weight: 235 aa, 26 kDa
• GenBank Accession Number: BC025715
• Gene Symbol: CD8A
• Gene ID (NCBI): 925
• Conjugate: APC-Cyanine7 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 778 nm
• Form: Liquid
• Purification Method: Unknow
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).