FcZero-rAb™ APC Anti-Human CD89 Rabbit Recombinant Antibody

• 

  FC experiment of human peripheral blood leukocytes using APC-FcA98105

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul APC Anti-Human CD89 Rabbit RecAb (APC-FcA98105, Clone:241426E3) or APC Rabbit IgG Isotype Control RecAb (APC-FcA98136, Clone: 240953C9). Cells were not fixed.

CD89 {ptg:Brand} Recombinant Antibody for FC

Cat No. APC-FcA98105

Clone No.241426E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

IgA Fc receptor, FCAR, 241426E3

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-FcA98105 targets CD89 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: Fc fragment of IgA, receptor for
• Calculated Molecular Weight: 287 aa, 32 kDa
• GenBank Accession Number: BC027953
• Gene Symbol: CD89
• Gene ID (NCBI): 2204
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

FcαRI (CD89) is a member of the Fc receptor immunoglobulin superfamily, constitutively expressed on cells of the myeloid lineage like neutrophils, eosinophils, monocytes macrophages, and platelets (PMID: 28103138). CD89 expression and activation are increased in human blood phagocytes following bacterial infection, together with increased expression and association of FcRγ with CD89 (PMID: 34756952). CD89 binds both subclasses of IgA (IgA1 and IgA2) with moderate affinity, but also binds other molecules such as TfR1, C-reactive protein and bacterial ligands. CD89 represents an effective target molecule for immunotherapy mediated by bispecific antibodies (PMID: 37081893).