CoraLite® Plus 647 Anti-Mouse CD86 Rabbit Recombinant Antibody

CD86 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

230476B8

Cat no : CL647-98025

Synonyms

B7-2, B70, B7.2, B7 2, B7

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Validation Data Gallery

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  FC experiment of mouse splenocytes using CL647-98025

  1x10^6 unstimulated (left) or LPS treated (right) mouse splenocytes were surface stained with 0.25ug CoraLite® Plus 647 Anti-Mouse CD86 Rabbit Recombinant Antibody (CL647-98025, Clone: 230476B8). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL647-98025

  1x10^6 LPS treated mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD86 Rabbit Recombinant Antibody (CL647-98025, Clone: 230476B8) (red) or Isotype Control (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD86 Rabbit Recombinant Antibody (CL647-98025, Clone: 230476B8) (black). Cells were not fixed.

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Tested Applications

• Positive FC detected in: LPS treated mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL647-98025 is CD86 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD86 antigen
• Calculated Molecular Weight: 35 kDa
• GenBank Accession Number: NM_019388
• Gene Symbol: Cd86
• Gene ID (NCBI): 12524
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)