CoraLite®594 Anti-Human CD86 (BU63)

CD86 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

BU63

Cat no : CL594-65165

Synonyms

CD28LG2, BU63, B70, B7 2, Activation B7-2 antigen

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Validation Data Gallery

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  FC experiment of human PBMCs using CL594-65165

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD86 (CL594-65165, Clone:BU63) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124, Clone: MOPC-21) and CL750 Anti-Human CD14 (CL750-65246). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using CL594-65165

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD86 (CL594-65165, Clone:BU63) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124, Clone: MOPC-21). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65165 targets CD86 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: B-lymphoblastoid cell line ARH 77
• Full Name: CD86 molecule
• Calculated Molecular Weight: 329 aa, 38 kDa
• GenBank Accession Number: BC040261
• Gene Symbol: CD86
• Gene ID (NCBI): 942
• RRID: AB_2883470
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.