CoraLite®594 Anti-Human CD86 (BU63)

CD86 Monoclonal Antibody for FC
Cat No. CL594-65165
Clone No.BU63

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

CD28LG2, BU63, B70, B7 2, Activation B7-2 antigen

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65165 targets CD86 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen B-lymphoblastoid cell line ARH 77 Predict reactive species
Full Name CD86 molecule
Calculated Molecular Weight 329 aa, 38 kDa
GenBank Accession NumberBC040261
Gene Symbol CD86
Gene ID (NCBI) 942
ENSEMBL Gene IDENSG00000114013
RRIDAB_2883470
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP42081
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

Protocols

Product Specific Protocols
FC protocol for CL594 CD86 antibody CL594-65165Download protocol
Standard Protocols
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