FITC Anti-Human CD83 (HB15e)

CD83 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1 kappa

Reactivity

Human, Non-human primates

Applications

FC

Conjugate

FITC Fluorescent Dye

CloneNo.

HB15e

Cat no : FITC-65171

Synonyms

B cell activation protein, BL11, CD83, CD83 antigen, CD83 molecule, Cell surface protein HB15, HB15, hCD83

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Validation Data Gallery

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  FC experiment of Human Monocytes-derived dendritic cells using FITC-65171

  1X10^6 Human monocytes-derived dendritic cells (induced with GM-CSF+IL-4+TNF-α) were surface stained with 4 ul FITC Anti-Human CD83 (FITC-65171, Clone:HB15e) (green) or FITC mouse IgG1 isotype control (black). Cells were not fixed.

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Tested Applications

• Positive FC detected in: Human monocytes-derived dendritic cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 4 µl per 10^6 cells in a 100 µl suspension or 4 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65171 targets CD83 in FC applications and shows reactivity with Human, Non-human primates samples.

• Tested Reactivity: Human, Non-human primates
• Host / Isotype: Mouse / Mouse IgG1 kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human CD83-transfected Cos cells
• Full Name: CD83 molecule
• Calculated Molecular Weight: 205 aa, 23 kDa
• GenBank Accession Number: BC030830
• Gene Symbol: CD83
• Gene ID (NCBI): 9308
• RRID: AB_2920503
• Conjugate: FITC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 494 nm / 520 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.