Anti-Human CD82 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98278-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD82 Rabbit Recombinant Antibody (98278-1-RR, Clone: 241765F7) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98278-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD82 Rabbit Recombinant Antibody (98278-1-RR, Clone: 241765F7) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

CD82 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98278-1-RR

Clone No.241765F7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD82, KAI1, Metastasis suppressor Kangai-1, IA4, CD82,KAI1, CD 82

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98278-1-RR targets CD82 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD82 molecule
• Calculated Molecular Weight: 30 kDa
• GenBank Accession Number: NM_002231.4
• Gene Symbol: CD82
• Gene ID (NCBI): 3732
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P27701-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.