CD82 Recombinant antibody, PBS Only

CD82 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241765G7

Cat no : 84617-7-PBS

Synonyms

CD82, KAI1, Metastasis suppressor Kangai-1, IA4, CD82,KAI1, CD 82

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Validation Data Gallery

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  WB analysis of Jurkat using 84617-7-RR (same clone as 84617-7-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 84617-7-RR (CD82 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84617-7-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 84617-7-RR (same clone as 84617-7-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 84617-7-RR (CD82 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84617-7-PBS in a different storage buffer formulation.

• 

  WB analysis of U-87 MG using 84617-7-RR (same clone as 84617-7-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 84617-7-RR (CD82 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84617-7-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 84617-7-RR (same clone as 84617-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84617-7-RR (CD82 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84617-7-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 84617-7-RR (same clone as 84617-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84617-7-RR (CD82 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84617-7-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84617-7-RR (same clone as 84617-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84617-7-RR (CD82 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84617-7-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84617-7-RR (same clone as 84617-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84617-7-RR (CD82 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84617-7-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84617-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84617-7-RR against Human CD82 were performed. The affinity constant is below 1 pM.

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Product Information

84617-7-PBS targets CD82 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD82 molecule
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 35-45 kDa
• GenBank Accession Number: NM_002231.4
• Gene Symbol: CD82
• Gene ID (NCBI): 3732
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.