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CD82 Recombinant antibody

CD82 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241765G7

Cat no : 84617-7-RR

Synonyms

CD82, KAI1, Metastasis suppressor Kangai-1, IA4, CD82,KAI1, CD 82

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD82 Recombinant antibody (84617-7-RR)

    WB analysis of Jurkat using 84617-7-RR

    Jurkat cells were subjected to SDS PAGE followed by western blot with 84617-7-RR (CD82 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using CD82 Recombinant antibody (84617-7-RR)

    WB analysis of Raji using 84617-7-RR

    Raji cells were subjected to SDS PAGE followed by western blot with 84617-7-RR (CD82 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-87 MG cells using CD82 Recombinant antibody (84617-7-RR)

    WB analysis of U-87 MG using 84617-7-RR

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 84617-7-RR (CD82 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CD82 Recombinant antibody (84617-7-RR)

    IHC staining of human ovary cancer using 84617-7-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84617-7-RR (CD82 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CD82 Recombinant antibody (84617-7-RR)

    IHC staining of human ovary cancer using 84617-7-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84617-7-RR (CD82 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD82 Recombinant antibody (84617-7-RR)

    IHC staining of human tonsillitis using 84617-7-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84617-7-RR (CD82 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD82 Recombinant antibody (84617-7-RR)

    IHC staining of human tonsillitis using 84617-7-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84617-7-RR (CD82 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84617-7-RR

    Affinity and Kinetic Characterization of 84617-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84617-7-RR against Human CD82 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inJurkat cells, Raji cells, U-87 MG cells
Positive IHC detected inhuman tonsillitis tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84617-7-RR targets CD82 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name CD82 molecule
Calculated Molecular Weight30 kDa
Observed Molecular Weight35-45 kDa
GenBank Accession NumberNM_002231.4
Gene Symbol CD82
Gene ID (NCBI) 3732
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.

Protocols

Product Specific Protocols
WB protocol for CD82 antibody 84617-7-RRDownload protocol
IHC protocol for CD82 antibody 84617-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols