Tested Applications
| Positive FC detected in | mouse peritoneal macrophages |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL750-65076 targets CD80 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CHO cell line transfected with mouse B7 (CD80) Predict reactive species |
| Full Name | CD80 antigen |
| GenBank Accession Number | BC131959 |
| Gene Symbol | CD80 |
| Gene ID (NCBI) | 12519 |
| RRID | AB_3084841 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Unknow |
| UNIPROT ID | Q00609 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 750 CD80 antibody CL750-65076 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
