CD79a Polyclonal antibody, PBS Only

CD79a Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22349-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 22349-1-AP (same clone as 22349-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22349-1-AP (CD79a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22349-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 22349-1-AP (same clone as 22349-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22349-1-AP (CD79a antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22349-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 22349-1-AP (same clone as 22349-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22349-1-AP (CD79A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22349-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 22349-1-AP (same clone as 22349-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22349-1-AP (CD79A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22349-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 22349-1-AP (same clone as 22349-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22349-1-AP (CD79A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22349-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 22349-1-AP (same clone as 22349-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD79a antibody (22349-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22349-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 22349-1-AP (same clone as 22349-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD79a antibody (22349-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22349-1-PBS in a different storage buffer formulation.

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Product Information

22349-1-PBS targets CD79a in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD79a fusion protein Ag17924
• Full Name: CD79a molecule, immunoglobulin-associated alpha
• Calculated Molecular Weight: 226 aa, 25 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC113733
• Gene Symbol: CD79A
• Gene ID (NCBI): 973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cell and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.