CD79a Polyclonal antibody
CD79a Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 22349-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Daudi cells, Raji cells, Ramos cells |
Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IHC | See 1 publications below |
Product Information
22349-1-AP targets CD79a in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CD79a fusion protein Ag17924 |
Full Name | CD79a molecule, immunoglobulin-associated alpha |
Calculated Molecular Weight | 226 aa, 25 kDa |
Observed Molecular Weight | 38-42 kDa |
GenBank Accession Number | BC113733 |
Gene Symbol | CD79A |
Gene ID (NCBI) | 973 |
RRID | AB_2879084 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cell and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.
Protocols
Product Specific Protocols | |
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WB protocol for CD79a antibody 22349-1-AP | Download protocol |
IHC protocol for CD79a antibody 22349-1-AP | Download protocol |
IF protocol for CD79a antibody 22349-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Int J Biol Macromol Structure characterization and intestinal immune promotion effect of polysaccharide purified from Alhagi camelorum Fisch |