Anti-Human CD79b Rabbit Recombinant Antibody

CD79b Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241273B7

Cat no : 98075-1-RR

Synonyms

IGB, Ig beta, B-cell-specific glycoprotein B29, B-cell antigen receptor complex-associated protein beta chain, B29

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Validation Data Gallery

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  FC experiment of human PBMCs using 98075-1-RR

  1x10^6 human PBMCs were stained with CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7) and 0.25 ug Anti-Human CD79b Rabbit Recombinant Antibody (98075-1-RR, Clone:241273B7), or Isotype Control. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98075-1-RR

  1x10^6 human PBMCs were stained with 0.25 ug Anti-Human CD79b Rabbit Recombinant Antibody (98075-1-RR, Clone:241273B7) (red), or Isotype Control(blue). Cells were not fixed.

• 

  FC experiment of human PBMCs using 98075-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD79b Rabbit Recombinant Antibody (98075-1-RR, Clone:241273B7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7), and 0.25 ug Isotype Control. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98075-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98075-1-RR against Human CD79b were performed. The affinity constant is 1.41 nM.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98075-1-RR targets CD79b in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: CD79b molecule, immunoglobulin-associated beta
• Calculated Molecular Weight: 229 aa, 26 kDa
• GenBank Accession Number: BC032651
• Gene Symbol: CD79B
• Gene ID (NCBI): 974
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD79 molecule encompasses two transmembrane proteins, CD79a and CD79b, which form a disulfide-linked heterodimer and are members of the immunoglobulin (Ig) gene superfamily (PMID: 2033258; 1375264; 2304550). CD79b molecule (also known as B29, IGB, AGM6, and Igbeta) is expressed almost exclusively on B cells and B-cell neoplasms, influencing antigen internalization and increasing antigen presentation efficiency (PMID: 7552998). CD79b mutation could be a key biomarker for DLBCL disease progression (PMID: 36182550).