CoraLite® Plus 488-conjugated CD74 Monoclonal antibody

CD74 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D8A1

Cat no : CL488-66390

Synonyms

Ia antigen-associated invariant chain, HLA-DR antigens-associated invariant chain, HLADG, DHLAG, Class-II-associated invariant chain peptide

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Validation Data Gallery

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  IF Staining of Raji using CL488-66390

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CoraLite® Plus 488 CD74 antibody (CL488-66390, Clone: 2D8A1 ) at dilution of 1:200.

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  FC experiment of human PBMCs using CL488-66390

  1x10^6 human PBMCs were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated CD74 Monoclonal antibody (CL488-66390, Clone: 2D8A1) or 0.4 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4). Cells were co-stained with 0.4 ug CoraLite® Plus 647 Anti-Human CD19 (SJ25C1) (CL647-65145, Clone: SJ25C1). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: Raji cells
• Positive FC (Intra) detected in: human PBMCs

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66390 targets CD74 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD74 fusion protein Ag25503
• Full Name: CD74 molecule, major histocompatibility complex, class II invariant chain
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC018726
• Gene Symbol: CD74
• Gene ID (NCBI): 972
• RRID: AB_2883326
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C