FITC Plus Anti-Human CD73 (AD2) Rabbit Recombinant Antibody

CD73 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

AD2

Cat no : FITC-65500

Synonyms

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73)



Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65500 targets CD73 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Pre-B leukemia cell line
Full Name 5'-nucleotidase, ecto (CD73)
Calculated Molecular Weight 29 kDa, 63 kDa
GenBank Accession NumberBC015940
Gene Symbol NT5E
Gene ID (NCBI) 4907
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

Protocols

Product Specific Protocols
FC protocol for FITC Plus CD73 antibody FITC-65500Download protocol
Standard Protocols
Click here to view our Standard Protocols