CoraLite® Plus 647 Anti-Human CD71 (OKT9) Rabbit Recombinant Antibody

CD71 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

OKT9

Cat no : CL647-65507

Synonyms

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

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Validation Data Gallery

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  FC experiment of human PBMCs using CL647-65507

  1X10^6 PHA-activated (3 day) human PBMCs were surface stained with FITC Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human CD71 Rabbit Recombinant Antibody (CL647-65507, Clone:OKT9) (right) or CoraLite® Plus 647-conjugated Rabbit IgG control (CL647-30000) (left). 1X10^6 unactivated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD71 Mouse Monoclonal Antibody (center). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65507

  1X10^6 PHA-activated (3 day) human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD71 Rabbit Recombinant Antibody (CL647-65507, Clone:OKT9) (red) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000) (blue). 1X10^6 unactivated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD71 Mouse Monoclonal Antibody (black dashed). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65507

  1X10^6 PHA-activated (3 day) human PBMCs were surface stained with FITC Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human CD71 Rabbit Recombinant Antibody (CL647-65507, Clone:OKT9) (left) or 5 ul CoraLite® Plus 647 Anti-Human CD71 Mouse Monoclonal Antibody (CL647-65235, Clone:OKT9) (right). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: PHA treated (3 day) human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65507 targets CD71 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Human T-cell acute lymphoblastic leukemia cells
• Full Name: transferrin receptor (p90, CD71)
• Calculated Molecular Weight: 85 kDa
• GenBank Accession Number: BC001188
• Gene Symbol: CD71
• Gene ID (NCBI): 7037
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.