CoraLite® Plus 594 Anti-Human CD71 (OKT9)

• 

  FC experiment of human PBMCs using CL594-65235

  1x10^6 PHA treated (3 days) human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD71 (CL594-65235, Clone:OKT9) (red) or CoraLite®594 Mouse IgG1 Isotype Control (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD71 (CL594-65235, Clone:OKT9) (black dashed). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL594-65235

  1x10^6 PHA treated (3 days) human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 594 Anti-Human CD71 (CL594-65235, Clone:OKT9) (right) or CoraLite®594 Mouse IgG1 Isotype Control (left). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD71 (CL594-65235, Clone:OKT9) (center). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

CD71 Monoclonal Antibody for FC

Cat No. CL594-65235

Clone No.OKT9

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Synonyms

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

---

Tested Applications

• Positive FC detected in: PHA treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65235 targets CD71 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human T-cell acute lymphoblastic leukemia cells
• Full Name: transferrin receptor (p90, CD71)
• Calculated Molecular Weight: 85 kDa
• GenBank Accession Number: BC001188
• Gene Symbol: CD71
• Gene ID (NCBI): 7037
• ENSEMBL Gene ID: ENSG00000072274
• RRID: AB_3673470
• Conjugate: CoraLite® Plus 594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 594 nm / 615 nm
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: P02786
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.