FC experiment of mouse bone marrow cells using 65654-1-TR
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD71 (RI7217) Rat IgG2a RecAb (65654-1-TR, Clone: RI7217) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3), and FITC anti-Rat IgG2a antibody. Cells were then stained with APC Anti-Mouse TER-119. Cells were not fixed.
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD71 (RI7217) Rat IgG2a RecAb (65654-1-TR, Clone: RI7217) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3), and FITC anti-Rat IgG2a antibody. Cells were then stained with APC Anti-Mouse TER-119. Cells were not fixed.
FC experiment of mouse bone marrow cells using 65654-1-TR
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD71 (RI7217) Rat IgG2a RecAb (65654-1-TR, Clone: RI7217) (red) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed.
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD71 (RI7217) Rat IgG2a RecAb (65654-1-TR, Clone: RI7217) (red) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
65654-1-TR targets CD71 in FC applications and shows reactivity with mouse samples.
Store at 2-8°C. Stable for one year after shipment.
Background Information
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule, creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
FC experiment of mouse bone marrow cells using 65654-1-TR
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD71 (RI7217) Rat IgG2a RecAb (65654-1-TR, Clone: RI7217) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3), and FITC anti-Rat IgG2a antibody. Cells were then stained with APC Anti-Mouse TER-119. Cells were not fixed.
FC experiment of mouse bone marrow cells using 65654-1-TR
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD71 (RI7217) Rat IgG2a RecAb (65654-1-TR, Clone: RI7217) (red) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD71 Recombinant antibody
Catalog Number
65654-1-TR
Citations
-
Dilutions
Applications
FC
Reactivity
mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
