Tested Applications
Positive FC detected in | U266 cells |
Recommended dilution
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
PE-FcA98098 targets CD70 in FC applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Recombinant Protein Predict reactive species |
Full Name | CD70 molecule |
Calculated Molecular Weight | 21 kDa |
GenBank Accession Number | BC000725 |
Gene Symbol | CD70 |
Gene ID (NCBI) | 970 |
Conjugate | PE Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD70 is the membrane-bound ligand of the CD27 receptor, which belongs to the tumor necrosis factor receptor superfamily. CD70 is not expressed in non-lymphoid normal tissue but is overexpressed in tumor cells of various solid cancers, diffuse large B-cell and follicular lymphoma and the malignant cells of Hodgkin lymphoma, Waldenström macroglobulinemia, and multiple myeloma (PMID: 28651374, 20699361 ). CD70/CD27 interactions play an important role in providing co-stimulation during the development of functional lymphocytes; strict control of CD70 expression is required for optimal signaling for immune cell activation (PMID: 21304103).
Protocols
Product Specific Protocols | |
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FC protocol for PE CD70 antibody PE-FcA98098 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |