CoraLite® Plus 647-conjugated CD70 Recombinant monoclonal antibody

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  IF Staining of Raji using CL647-84455-2

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CoraLite® Plus 647 CD70 antibody (CL647-84455-2, Clone: 241324B12 ) at dilution of 1:200.

CD70 Recombinant Antibody for IF/ICC

Cat No. CL647-84455-2

Clone No.241324B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

241324B12, CD27 L, CD27 ligand, CD27L, CD27-L

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Tested Applications

• Positive IF/ICC detected in: Raji cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-84455-2 targets CD70 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD70 molecule
• Calculated Molecular Weight: 21 kDa
• GenBank Accession Number: BC000725
• Gene Symbol: CD70
• Gene ID (NCBI): 970
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P32970
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD70, also known as tumor necrosis factor ligand superfamily member 7 (TNFSF7) or CD27 ligand (CD27L), is a transmembrane protein that plays a significant role in the immune system. It is the unique ligand for CD27, a member of the tumor necrosis factor receptor (TNFR) family, and is transiently upregulated upon stimulation of immune cells. CD70 is expressed on lymphocytes and dendritic cells, and its expression can be regulated by activation signals received via toll-like receptors (TLRs), CD40, and the antigen receptor MHC II. It provides complex and not completely understood signaling for B cell development. CD70 is also expressed on various solid tumors and hematolymphoid malignancies, where it may contribute to a poor prognosis.