CD7 Monoclonal antibody, PBS Only

CD7 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A4E6

Cat no : 60209-2-PBS

Synonyms

T cell leukemia antigen, T cell antigen CD7, LEU-9, GP40, CD7 molecule

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Validation Data Gallery

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  WB analysis of Jurkat using 60209-2-Ig (same clone as 60209-2-PBS)

  Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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  WB analysis using 60209-2-Ig (same clone as 60209-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-2-Ig(CD7 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-2-Ig(CD7 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse thymus using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse thymus using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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Product Information

60209-2-PBS targets CD7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD7 fusion protein Ag1805
• Full Name: CD7 molecule
• Calculated Molecular Weight: 240 aa, 25 kDa
• Observed Molecular Weight: 34-36 kDa
• GenBank Accession Number: BC009293
• Gene Symbol: CD7
• Gene ID (NCBI): 924
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD7 is a type I transmembrane glycoprotein expressed on thymocytes and mature T cells. It is the most reliable clinical marker of T-cell acute lymphocytic leukemia.