Filter: AllWBIHC

WB analysis of Jurkat using 60209-2-Ig (same clone as 60209-2-PBS)
Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.
WB analysis using 60209-2-Ig (same clone as 60209-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 60209-2-Ig (same clone as 60209-2-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 60209-2-Ig (same clone as 60209-2-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.
IHC staining of mouse thymus using 60209-2-Ig (same clone as 60209-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.
IHC staining of mouse thymus using 60209-2-Ig (same clone as 60209-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 60209-2-Ig (same clone as 60209-2-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-2-Ig(CD7 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 60209-2-Ig (same clone as 60209-2-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-2-Ig(CD7 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

CD7 Monoclonal antibody, PBS Only

CD7 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 60209-2-PBS

Clone No.2A4E6

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

T cell leukemia antigen, T cell antigen CD7, LEU-9, GP40, CD7 molecule

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60209-2-PBS targets CD7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CD7 fusion protein Ag1805 Predict reactive species
Full Name	CD7 molecule
Calculated Molecular Weight	240 aa, 25 kDa
Observed Molecular Weight	34-36 kDa
GenBank Accession Number	BC009293
Gene Symbol	CD7
Gene ID (NCBI)	924
ENSEMBL Gene ID	ENSG00000173762
RRID	AB_2881355
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P09564
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

CD7 is a type I transmembrane glycoprotein expressed on thymocytes and mature T cells. It is the most reliable clinical marker of T-cell acute lymphocytic leukemia.