FITC Plus Anti-Human CD68 (KP1) Mouse IgG2a Recombinant Antibody

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  FC experiment of human PBMCs using FITC-65593

  1x10^6 human PBMCs were intracellularly stained with APC Anti-Human CD14 and 5 ul FITC Plus Anti-Human CD68 (KP1) Mouse IgG2a RecAb (FITC-65593, Clone: KP1) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human PBMCs using FITC-65593

  1x10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 (KP1) Mouse IgG2a RecAb (FITC-65593, Clone: KP1) (red) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CD68 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. FITC-65593

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra)

Synonyms

SCARD1, Macrosialin, KP1, GP110, CD68 molecule

Formulation:  PBS and Azide

PBS and Azide

Conjugate:  FITC Plus

Unconjugated Biotin CoraLite® Plus 405 CoraLite® Plus 647 CoraLite® Plus 750 CoraLite®594 FITC Plus

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Tested Applications

• Positive FC (Intra) detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65593 targets CD68 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: CD68 molecule
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: BC015557
• Gene Symbol: CD68
• Gene ID (NCBI): 968
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.