CD68 Monoclonal antibody, PBS Only

CD68 Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A9A7

Cat no : 66231-2-PBS

Synonyms

CD68, CD68 molecule, GP110, Macrosialin, SCARD1

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Validation Data Gallery

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  IHC staining of human tonsillitis using 66231-2-Ig (same clone as 66231-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66231-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66231-2-Ig (same clone as 66231-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66231-2-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 66231-2-Ig (same clone as 66231-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66231-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66231-2-Ig (same clone as 66231-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66231-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66231-2-Ig (same clone as 66231-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66231-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 66231-2-Ig (same clone as 66231-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66231-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 66231-2-Ig (same clone as 66231-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66231-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66231-2-Ig (same clone as 66231-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66231-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66231-2-Ig (same clone as 66231-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66231-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66231-2-Ig (same clone as 66231-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66231-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66231-2-Ig (same clone as 66231-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66231-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66231-2-Ig (same clone as 66231-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66231-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66231-2-Ig (same clone as 66231-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66231-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66231-2-Ig (same clone as 66231-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66231-2-Ig (CD68 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66231-2-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66231-2-Ig (same clone as 66231-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD68 antibody (66231-2-Ig, Clone: 3A9A7 ) at dilution of 1:6000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66231-2-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66231-2-Ig (same clone as 66231-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD68 antibody (66231-2-Ig, Clone: 3A9A7 ) at dilution of 1:6000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66231-2-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66231-2-PBS targets CD68 in IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD68 fusion protein Ag22815
• Full Name: CD68 molecule
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: BC015557
• Gene Symbol: CD68
• Gene ID (NCBI): 968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.