Anti-Human CD68 (KP1) Rabbit IgG Recombinant Antibody

CD68 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, FC (Intra)

Conjugate

Unconjugated

Publications

1

CloneNo.

KP1

Cat no : 65515-1-RR

Synonyms

CD68, CD68 molecule, GP110, KP1, Macrosialin, SCARD1

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Validation Data Gallery

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  FC experiment of human PBMCs using 65515-1-RR

  1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-RR, Clone:KP1) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65515-1-RR

  1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-RR, Clone:KP1) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65515-1-RR

  1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-RR, Clone:KP1) (red) and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.1 ug Anti-Human CD68 Mouse Monoclonal Antibody (Clone:KP1) (65202-1-Ig in green; or from another vendor in orange) and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated.

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Tested Applications

• Positive FC (Intra) detected in: human PBMCs
• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

65515-1-RR targets CD68 in WB, IHC, FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Lysosomal fraction of human alveolar macrophages
• Full Name: CD68 molecule
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: BC015557
• Gene Symbol: CD68
• Gene ID (NCBI): 968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Publications

Species	Application	Title
human	IHC,WB	Gene; Potential predictive value of immune-related genes FUCA1 and NCKAP1L for osteosarcoma metastasis; Authors - Xuan Wang