1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-PBS, Clone:KP1) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated.
1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-PBS, Clone:KP1) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated.
FC experiment of human PBMCs using 65515-1-RR (same clone as 65515-1-PBS)
1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-RR, Clone:KP1) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated. This data was developed using the same antibody clone with 65515-1-PBS in a different storage buffer formulation.
1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-RR, Clone:KP1) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated. This data was developed using the same antibody clone with 65515-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65515-1-RR (same clone as 65515-1-PBS)
1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-RR, Clone:KP1) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated. This data was developed using the same antibody clone with 65515-1-PBS in a different storage buffer formulation.
1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-RR, Clone:KP1) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated. This data was developed using the same antibody clone with 65515-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65515-1-PBS
1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-PBS, Clone:KP1) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated.
1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-PBS, Clone:KP1) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated.
FC experiment of human PBMCs using 65515-1-PBS
1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-PBS, Clone:KP1) (red) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.1 ug Anti-Human CD68 Mouse Monoclonal Antibody (Clone:KP1) (65202-1-Ig in green; or from another vendor in orange) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated.
1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-PBS, Clone:KP1) (red) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.1 ug Anti-Human CD68 Mouse Monoclonal Antibody (Clone:KP1) (65202-1-Ig in green; or from another vendor in orange) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated.
FC experiment of human PBMCs using 65515-1-RR (same clone as 65515-1-PBS)
1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-RR, Clone:KP1) (red) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.1 ug Anti-Human CD68 Mouse Monoclonal Antibody (Clone:KP1) (65202-1-Ig in green; or from another vendor in orange) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated. This data was developed using the same antibody clone with 65515-1-PBS in a different storage buffer formulation.
1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-RR, Clone:KP1) (red) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.1 ug Anti-Human CD68 Mouse Monoclonal Antibody (Clone:KP1) (65202-1-Ig in green; or from another vendor in orange) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated. This data was developed using the same antibody clone with 65515-1-PBS in a different storage buffer formulation.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Product Information
65515-1-PBS targets CD68 in FC (Intra) applications and shows reactivity with human samples.
Store at -80°C. Stable for one year after shipment.
Background Information
CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.
1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-PBS, Clone:KP1) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated.
FC experiment of human PBMCs using 65515-1-RR (same clone as 65515-1-PBS)
1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-RR, Clone:KP1) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated. This data was developed using the same antibody clone with 65515-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65515-1-RR (same clone as 65515-1-PBS)
1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-RR, Clone:KP1) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated. This data was developed using the same antibody clone with 65515-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65515-1-PBS
1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-PBS, Clone:KP1) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated.
FC experiment of human PBMCs using 65515-1-PBS
1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-PBS, Clone:KP1) (red) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.1 ug Anti-Human CD68 Mouse Monoclonal Antibody (Clone:KP1) (65202-1-Ig in green; or from another vendor in orange) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated.
FC experiment of human PBMCs using 65515-1-RR (same clone as 65515-1-PBS)
1x10^6 human PBMCs were intracellularly stained with 0.1 ug Anti-Human CD68 Rabbit Recombinant Antibody (65515-1-RR, Clone:KP1) (red) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.1 ug Anti-Human CD68 Mouse Monoclonal Antibody (Clone:KP1) (65202-1-Ig in green; or from another vendor in orange) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) and treated with FC Receptor Block prior to staining. Monocytes were gated. This data was developed using the same antibody clone with 65515-1-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
