Biotin Anti-Human CD64 (10.1) Mouse IgG2a Recombinant Antibody

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  FC experiment of human PBMCs using Biotin-65572

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD64 (10.1) Mouse IgG2a RecAb (Biotin-65572, Clone:10.1) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with APC Anti-Human CD14 Rabbit Recombinant Antibody (APC-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-65572

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD64 (10.1) Mouse IgG2a RecAb (Biotin-65572, Clone:10.1) and CoraLite®488-conjugated streptavidin (red) or unstained (blue).Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD64 Uni-rAb^TM Recombinant Antibody for FC

Cat No. Biotin-65572

Clone No.10.1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

Fc-gamma RI, FCG1, Fc gamma RIA, Fc gamma RI, CD64A

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65572 targets CD64 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
• Full Name: Fc fragment of IgG, high affinity Ia, receptor (CD64)
• Calculated Molecular Weight: 374 aa, 43 kDa
• GenBank Accession Number: BC032634
• Gene Symbol: FCGR1A
• Gene ID (NCBI): 2209
• ENSEMBL Gene ID: ENSG00000150337
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.