CD63 Monoclonal antibody

CD63 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (3)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

43

CloneNo.

3D4D1

Reviews (1)

Cat no : 67605-1-Ig

Synonyms

CD63, CD63 antigen, CD63 molecule, Granulophysin, ME491, MLA1, OMA81H, Tetraspanin 30, Tspan 30, TSPAN30

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Validation Data Gallery

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  WB analysis using 67605-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67605-1-Ig (CD63 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 67605-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67605-1-Ig (CD63 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 67605-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:700 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67605-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:700 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67605-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67605-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67605-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67605-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 67605-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human malignant melanoma using 67605-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:500 (under 40x lens).

• 

  IF Staining of human tonsillitis using 67605-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 67605-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human lymphoma using 67605-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human lymphoma using 67605-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 67605-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human malignant melanoma using 67605-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human malignant melanoma using 67605-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of THP-1 using 67605-1-Ig

  1X10^6 THP-1 cells were surface stained with 0.4 ug Anti-Human CD63 (67605-1-Ig, Clone:3D4D1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed .

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Tested Applications

• Positive WB detected in: HeLa cells, U2OS cells, MCF-7 cells, A549 cells, K-562 cells, HL-60, THP-1 cells, LNCaP cells, HUVEC cells
• Positive IHC detected in: human tonsillitis tissue, human malignant melanoma tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human tonsillitis tissue, human malignant melanoma tissue, human lymphoma tissue
• Positive FC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:10000
• Immunohistochemistry (IHC): IHC : 1:350-1:1400
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 42 publications below
• FC: See 2 publications below

Product Information

67605-1-Ig targets CD63 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, rat, goat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD63 fusion protein Ag19690
• Full Name: CD63 molecule
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC002349
• Gene Symbol: CD63
• Gene ID (NCBI): 967
• RRID: AB_2882811
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediate signal transduction events that play a role in the regulation of cell development, activation and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.

Publications

Species	Application	Title
human	WB	Drug Des Devel Ther; Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling; Authors - Huan Hu
	WB	Oxid Med Cell Longev; Adipose-Derived Circulating Exosomes Promote Protection of the Pulmonary Endothelial Barrier by Inhibiting EndMT and Oxidative Stress through Down-Regulation of the TGF-β Pathway: A Potential Explanation for the Obesity Paradox in ARDS.; Authors - Di Qi
human	WB	Aging (Albany NY); MSC-derived exosomes promote recovery from traumatic brain injury via microglia/macrophages in rat.; Authors - Yunfei Chen
human	WB	Aging (Albany NY); Exosomal circRNAs contribute to intestinal development via the VEGF signalling pathway in human term and preterm colostrum.; Authors - Yahui Zhou
	WB	Aging (Albany NY); Endothelial cell-derived exosomal circHIPK3 promotes the proliferation of vascular smooth muscle cells induced by high glucose via the miR-106a-5p/Foxo1/Vcam1 pathway.; Authors - Shaohua Wang
	WB	Int J Mol Sci; MicroRNAs in Small Extracellular Vesicles from Amniotic Fluid and Maternal Plasma Associated with Fetal Palate Development in Mice; Authors - Xige Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guorong (Verified Customer) (03-22-2022)

Two bands at 30 and 60 kDa was detected

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: ARPE-19

CD63 Antibody Western Blot validation (1:5000 dilution) in ARPE-19 (Cat no:67605-1-Ig)