Anti-Human CD63 (H5C6)

CD63 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

H5C6

Cat no : 65295-1-Ig

Synonyms

Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, H5C6, CD63 antigen

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Validation Data Gallery

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  FC experiment of human peripheral blood platelets using 65295-1-Ig

  100ul thrombin-activated (red) or unactivated (black dashed) human peripheral blood platelets (diluted 1:2 in PBS) were surface stained with 0.2 ug Anti-Human CD63 (65295-1-Ig, Clone: H5C6) or Mouse IgG1 Isotype Control (65124-1-Ig) (blue) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed.

• 

  FC experiment of human peripheral blood platelets using 65295-1-Ig

  100ul thrombin-activated (red) or unactivated (black dashed) human peripheral blood platelets (diluted 1:2 in PBS) were surface stained with 0.2 ug Anti-Human CD63 (65295-1-Ig, Clone: H5C6) or Mouse IgG1 Isotype Control (65124-1-Ig) (blue) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed.

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Tested Applications

• Positive FC detected in: thrombin-activated human peripheral blood platelets

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65295-1-Ig targets CD63 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human splenic adherent cells
• Full Name: CD63 molecule
• GenBank Accession Number: BC013017
• Gene Symbol: CD63
• Gene ID (NCBI): 967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.