PerCP-Cyanine5.5 Anti-Human CD62L (DREG56)

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  FC experiment of human PBMCs using CPY5-65167

  1x10^6 human PBMCs were surface stained with 5 ul PerCP-Cyanine5.5 Anti-Human CD62L (DREG56) (CPY5-65167, Clone:DREG56) or PerCP-Cyanine5.5 Mouse IgG1 Isotype Control (MOPC-21) (CPY5-65124, Clone: MOPC-21). Cells were co-stained with FITC Plus Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CPY5-65167

  1x10^6 human PBMCs were surface stained with 5 ul PerCP-Cyanine5.5 Anti-Human CD62L (DREG56) (CPY5-65167, Clone: DREG56) (red) or PerCP-Cyanine5.5 Mouse IgG1 Isotype Control (MOPC-21) (CPY5-65124, Clone: MOPC-21) (blue). Cells were not fixed. Lymphocytes were gated.

CD62L Monoclonal Antibody for FC

Cat No. CPY5-65167

Clone No.DREG56

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Synonyms

L-selectin, L selectin, hLHRc, gp90-MEL, gp90 MEL

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CPY5-65167 targets CD62L in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: PMA-activated human peripheral blood leukocytes
• Full Name: selectin L
• Calculated Molecular Weight: 42 kDa
• GenBank Accession Number: BC020758
• Gene Symbol: CD62L
• Gene ID (NCBI): 6402
• ENSEMBL Gene ID: ENSG00000188404
• Conjugate: PerCP-Cyanine5.5 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 482 nm / 690nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P14151
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).