PE Anti-Mouse CD62L (L-Selectin) (MEL-14)

CD62L (L-Selectin) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

MEL-14

Cat no : PE-65123

Synonyms

CD62L (L-Selectin), Sell

---

Validation Data Gallery

• 

  FC experiment of mouse splenocytes using PE-65123

  1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and 0.13 ug PE-Anti-Mouse CD62L (L-Selectin) (PE-65123, Clone: MEL-14). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using PE-65123

  1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and 0.13 ug PE-Rat IgG2a isotype control antibody. Cells were not fixed.

---

Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65123 targets CD62L (L-Selectin) in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: C3H/eb mouse B lymphoma 38C-13
• Full Name: selectin, lymphocyte
• GenBank Accession Number: BC052681
• Gene Symbol: Sell
• Gene ID (NCBI): 20343
• RRID: AB_2883911
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).