CoraLite® Plus 488-conjugated CD59 Monoclonal antibody

CD59 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E3A1

Cat no : CL488-68222

Synonyms

HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 20 kDa homologous restriction factor

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Validation Data Gallery

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  IF Staining of human placenta using CL488-68222

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite® Plus 488 CD59 antibody (CL488-68222, Clone: 1E3A1 ) at dilution of 1:200.

• 

  IF Staining of human placenta using CL488-68222

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite® Plus 488 CD59 antibody (CL488-68222, Clone: 1E3A1 ) at dilution of 1:200.

• 

  IF Staining of BxPC-3 using CL488-68222

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using CoraLite® Plus 488 CD59 antibody (CL488-68222, Clone: 1E3A1 ) at dilution of 1:200.

• 

  IF Staining of BxPC-3 using CL488-68222

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using CoraLite® Plus 488 CD59 antibody (CL488-68222, Clone: 1E3A1 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human placenta tissue
• Positive IF/ICC detected in: BxPC-3 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68222 targets CD59 in IF/ICC, IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD59 fusion protein Ag32834
• Full Name: CD59 molecule, complement regulatory protein
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 18-25 kDa
• GenBank Accession Number: BC001506
• Gene Symbol: CD59
• Gene ID (NCBI): 966
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.