CD59 Monoclonal antibody, PBS Only

CD59 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E3A1

Cat no : 68222-1-PBS

Synonyms

16.3A5, 1F5, 1F5 antigen, CD59, CD59 glycoprotein, EJ16, EJ30, EL32, FLJ38134, FLJ92039, G344, HRF 20, HRF20, MAC inhibitory protein, MAC IP, MACIF, MEM43, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18 20, Protectin

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Validation Data Gallery

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  WB analysis using 68222-1-Ig (same clone as 68222-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68222-1-Ig (CD59 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  WB analysis using 68222-1-Ig (same clone as 68222-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68222-1-Ig (CD59 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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  IF Staining of BxPC-3 using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68222-1-PBS targets CD59 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD59 fusion protein Ag32834
• Full Name: CD59 molecule, complement regulatory protein
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 18-25 kDa
• GenBank Accession Number: BC001506
• Gene Symbol: CD59
• Gene ID (NCBI): 966
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.